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jccui82

1. Brookes P C，Powlson D S，Jenkinson D S．Measurement of microbial biomass phosphorus in soil [J]．Soil Biol．Biochem.，1982，14：319-329．
2.Hedley M J，Stewart J W．Method to measure microbial phosphate in soils [J]．Soil Biol. Biochem.，1982，14：377-385．

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Measurement of microbial biomass phosphorus in soil

Abstract
A method for measuring the amount of P held in soil micro-organisms (biomass P) is described and the assumptions on which it is based are discussed. Biomass P is calculated from the difference between the amount of inorganic P (Pi) extracted by 0.5 (Spm) NaHCO3 (pH 8.5) from fresh soil fumigated with CHCl3 and the amount extracted from unfumigated soil. Some CHCl3-released Pi is sorbed by soil during fumigation and extraction: an approximate allowance for this is made by incorporating a known quantity of Pi during extraction and correcting for recovery. Most of the P released is in inorganic form and the proportion increases with duration of fumigation. Non-microbial P is little, if at all, affected by fumigation. Microbial biomass P is calculated from CHCl3-released Pi by dividing by 0.4, i.e. by assuming that 40% of the P in the biomass is rendered extractable as Pi by CHCl3. Measurements of biomass P must be done in fresh soil, CHCl3 releases much less P in air-dry soil.

Method to measure microbial phosphate in soils

Abstract
The amount of phosphate in soil microbial biomass was estimated by adding CHCl3 to soil to lyse microbial cells and measuring the proportion of microbial-P released to 0.5 M NaHCO3 extracts (pH 8.5). Calculations of total microbial-P were based on the difference between P removed by NaHCO3 extraction ofChCl3 treated and untreated samples. This method was improved by (1) removing resin-extractable P from the soil before lysing microbial cells with CHCl3, (2) measuring total P rather than inorganic-P in NaHCO3 extracts, and (3) prolonging NaHCO3 extraction from 30 min to 16 h. The efficiency of extraction of soil microbial-P was determined by measuring the P recovered from two species each of bacteria and fungi grown first in solutions having different P concentrations, then added to soil. The average proportion of microbial-P recovered from a neutral calcareous soil was 37% (KP = 0.37). The Kp factor was confirmed by adding 33P-labelled bacteria to a similar soil and measuring the added 33P recovered (38%) by the CHCl3-NaHCO3 method.

jccui82

想要全文，谢谢

ethanliu

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